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Foreword xix
Acknowledgments xxi
Abbreviations xxiii
Book Navigation xxix
Part I Understanding Cell Culture
1. Introduction
2. Biology of Cultured Cells
3. Origin and Evolution of Cultured Cells
Part II Laboratory and Regulatory Requirements
4. Laboratory Design and Layout
5. Equipment and Materials
6. Safety and Bioethics
7. Reproducibility and Good Cell Culture Practice
Part III Medium and Substrate Requirements
8. Culture Vessels and Substrates
9. Defined Media and Supplements
10. Serum-Free Media
11. Preparation and Sterilization
Part IV Handling Cultures
12. Aseptic Technique
13. Primary Culture
14. Subculture and Cell Lines
15. Cryopreservation and Banking
Part V Validation and Characterization
16. Microbial Contamination
17. Cell Line Misidentification and Authentication
18. Cell Line Characterization
19. Quantitation and Growth Kinetics
Part VI Physical and Genetic Manipulation
20. Cell Cloning and Selection
21. Cell Separation and Sorting
22. Genetic Modification and Immortalization
Part VII Stem Cells and Differentiated Cells
23. Culture of Stem Cells
24. Culture of Specific Cell Types
25. Culture of Tumor Cells
26. Differentiation
Part VIII Model Environments and Applications
27. Three-Dimensional Culture
28. Scale-Up and Automation
29. Toxicity Testing
Part IX Teaching and Troubleshooting
30. Training
31. Problem Solving
32. In Conclusion
Appendix A Glossary
Appendix B Calculations and Preparation of Reagents
Appendix C Media Formulations
References
Index
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