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Contributors
Preface
1. Introduction to Functional Analysis of the Yeast Genome
SG Oliver
2. Whole Chromosome Analysis
EJ Louis
3. The Bioinformatics of the Yeast Genome
H-W Mewes
D Frishman A Zollner
K Heumann
4. Transformation of Yeast by the Lithium
Acetate/Single-Stranded Carrier DNA/PEG Method RD Gietz
RA Woods
5. PCR-Based Gene Targeting in Saccharomyces
cerevisiae A Wach
A Brachat
C Rebischung S Steiner
K Pokorni
S te Heesen
P Philippsen
6. Studying Essential Genes: Generating and
Using Promoter Fusions and Conditional Alleles
MJR Stark
7. Ty Mutagenesis
DJ Garfinkel
MJ Curcio
V Smith
8. Transcript Analysis AJP Brown
LM Furness
D Bailey
9. Reporter Genes and their Use in Studying Yeast Gene Expression
N Koloteva
JMX Hughes
JEG McCarthy
10. Transposon Tagging I: A Novel System
for Monitoring Protein Production, Function
and Localization P Ross-Macdonald
A Sheehan
C Friddle GS Roeder
M Snyder
11. Transposon Tagging II: Exploration of
Gene Function and Regulatory Networks in
Yeast with the Mini-Mu Transposon
M Bolotin-Fukuhara
12. Immunological Approaches to the Study of
Protein Localization in Yeast
IM Hagan
CJ Stirling
13. Posttranslational Modifications of Secretory Proteins
FM Klis
AFJ Ram
RC Montijn JC Kapteyn
LHP Caro
JH Vossen
MAA Van Berkel SSC Brekelmans
H Van den Ende
14. Studying Posttranslational Modifications
in Yeast: Intracellular Proteins
MJR Stark
15. Two-Hybrid Analysis of Protein-Protein
Interactions in Yeast J Rosamond
16. "Smart" Genetic Screens
J de la Cruz
M-C Daugeron
P Linder
17. Metabolic Control Analysis as a Tool in the Elucidation of the Function of Novel Genes
B Teusink
F Baganz
HV Westerhoff
SG Oliver
18. Identifying Stress Genes
WH Mager
K Maurer PW Piper
19. Identifying Genes Encoding Components of
the Protein Synthesis Machinery of the Yeast
Saccharomyces cerevisiae
MF Tuite
I Stansfield RJ Planta
20. MAP Kinase-Mediated Signal Transduction Pathways
M Molina
H Martin
M Sanchez
C Nombela
21. Analysis of the Candida albicans Genome
PT Magee
22. Applications of Yeast in the Discovery of Human Gene Function
PA Moore
23. Yeast Mutant and Plasmid Collections K-D Entian
P Kotter
24. Yeast Gene Analysis in the Next
Millennium AJP Brown
MF Tuite
Appendix I: The Ribosomal Protein Genes of
Saccharomyces cerevisiae RJ Planta
I Stansfield
MF Tuite
Appendix II: Yeast Growth AJP Brown
MF Tuite
Appendix III: Useful World Wide Web Addresses for Yeast Researchers
AJP Brown
Appendix IV: Nomenclature for Yeast Genes and Proteins
MF Tuite
Index

Yeast Gene Analysis, edited by two leading experts in the field, presents the most appropriate, up-to-date experimental approaches in detail. The layout is structured for ease of reference, and the volume will be essential reading for all researchers working in genomics, molecular biology, genetics, and microbiology. It provides a carefully selected collection of cutting-edge chapters reviewing alternative experimental approaches for the biologist wishing to study the function of genes identified by genome sequencing. A multi-authored book, it brings together experts from various areas of cell and molecular biology who provide insight into the analysis of genes in yeast.


---From the Publisher