-
외국도서
-
대학교재/전문서적
-
자연과학 일반
Protein Protocols Handbook
2013년 9월 9일 이후 누적수치입니다.
| 정가 |
120,000원 |
|---|
-
120,000원
3,600P (3%적립)
| 할인혜택 | |
|---|---|
| 적립혜택 |
|
|
|
| 추가혜택 |
|
-
이벤트/기획전
-
연관도서
-
상품권
목차
| Preface | |
| Contributors | |
| Protein Determination by UV Absorption | p. 3 |
| The Lowry Method for Protein Quantitation | p. 7 |
| The Bicinchoninic Acid (BCA) Assay for Protein Quantitation | p. 11 |
| The Bradford Method for Protein Quantitation | p. 15 |
| Ultrafast Protein Determinations Using Microwave Enhancement | p. 21 |
| Quantitation of Tryptophan in Proteins | p. 29 |
| Protein Quantitation Using Flow Cytometry | p. 33 |
| Copper Iodide Staining of Proteins on Solid Phases | p. 39 |
| Kinetic Silver Staining of Proteins Adsorbed to Microtiter Plates | p. 45 |
| Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins | p. 51 |
| SDS Polyacrylamide Gel Electrophoresis of Proteins | p. 55 |
| Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins | p. 63 |
| Cetyltrimethylammonium Bromide Discontinuous Gel Electrophoresis of Proteins: M[subscript r]-Based Separation of Proteins with Retained Native Activity | p. 67 |
| Acetic Acid-Urea Polyacrylamide Gel Electrophoresis of Basic Proteins | p. 83 |
| Acid-Urea-Triton Polyacrylamide Gels for Histones | p. 91 |
| SDS-Polyacrylamide Gel Electrophoresis of Peptides | p. 101 |
| Isoelectric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels | p. 109 |
| Isoelectric Focusing Under Denaturing Conditions | p. 115 |
| Radioisotopic Labeling of Proteins for Polyacrylamide Gel Electrophoresis | p. 121 |
| Two-Dimensional PAGE Using Carrier Ampholyte pH Gradients in the First Dimension | p. 127 |
| Two-Dimensional PAGE Using Immobilized pH Gradients | p. 133 |
| Two-Dimensional PAGE Using Flat-Bed IEF in the First Dimension | p. 139 |
| Free Zone Capillary Electrophoresis | p. 143 |
| Capillary Isoelectric Focusing with Electro-Osmotic Flow Mobilization | p. 155 |
| Quantification of Radiolabeled Proteins in Polyacrylamide Gels | p. 161 |
| Quantification of Proteins by Staining in Polyacrylamide Gels | p. 167 |
| Quantitation of Proteins Separated by Electrophoresis Using Coomassie Brilliant Blue | p. 173 |
| Rapid Staining of Proteins in Polyacrylamide Gels with Nile Red | p. 179 |
| Zn[superscript 2+]-Reverse Staining of Proteins in Polyacrylamide Gels | p. 187 |
| Protein Staining with Calconcarboxylic Acid in Polyacrylamide Gels | p. 197 |
| Detection of Proteins and Sialoglycoproteins in Polyacrylamide Gels Using Eosin Y Stain | p. 203 |
| Electroelution of Proteins form Polyacrylamide Gels | p. 207 |
| High-Performance Electrophoresis Chromatography | p. 215 |
| Drying Polyacrylamide Gels | p. 223 |
| Detection of Proteins in Polyacrylamide Gels by Silver Staining | p. 229 |
| Autoradiography and Flourography of Acrylamide Gels | p. 235 |
| Protein Blotting by Electroblotting | p. 245 |
| Protein Blotting by the Semidry Method | p. 249 |
| Protein Blotting by the Capillary Method | p. 261 |
| Protein Blotting of Basic Proteins Resolved on Acid-Urea-Triton-Polyacrylamide Gels | p. 263 |
| Alkaline Phosphatase Labeling of Antibody Using Glutaraldehyde | p. 269 |
| [Beta]-Galactosidase Labeling of Antibody Using MBS | p. 271 |
| Horseradish Peroxidase Labeling of Antibody Using Periodate Oxidation | p. 273 |
| Protein Staining and Immunodetection Using Colloidal Gold | p. 275 |
| Fluorescent Protein Staining on Nitrocellulose with Subsequent Immunodetection of Antigen | p. 289 |
| Coupling of Antibodies with Biotin | p. 293 |
| Preparation of Avidin Conjugates | p. 303 |
| Detection of Polypeptides on Blots Using Secondary Antibodies or Protein A | p. 313 |
| Detection of Proteins on Blots Using Avidin- or Streptavidin Biotin | p. 323 |
| Detection of Proteins on Blots Using Chemiluminescent Systems | p. 329 |
| Carboxymethylation of Cysteine Using Iodoacetamide/Iodoacetic Acid | p. 339 |
| Performic Acid Oxidation | p. 341 |
| Succinylation of Proteins | p. 343 |
| Pyridylethylation of Cysteine Residues | p. 345 |
| Side-Chain Selective Chemical Modifications of Proteins | p. 349 |
| Nitration of Tyrosines | p. 353 |
| Ethoxyformylation of Histidine | p. 357 |
| Modification of Arginine Side Chains with p-Hydroxyphenylglyoxal | p. 359 |
| Amidation of Carboxyl Groups | p. 361 |
| Amidination of Lysine Side Chains | p. 363 |
| Modification of Tryptophan with 2-Hydroxy-5-Nitrobenzylbromide | p. 365 |
| Modification of Sulfhydryl Groups with DTNB | p. 367 |
| Chemical Cleavage of Proteins at Methionyl Residues | p. 369 |
| Chemical Cleavage of Proteins at Tryptophan Residues | p. 375 |
| Chemical Cleavage of Proteins at Aspartyl Residues | p. 381 |
| Chemical Cleavage of Proteins at Cysteinyl Residues | p. 385 |
| Chemical Cleavage of Proteins at Asparaginyl-Glycyl Peptide Bonds | p. 389 |
| Preparation of Peptides for Microsequencing from Proteins in Polyacrylamide Gels | p. 393 |
| In Situ Chemical and Enzymatic Digestions of Proteins Immobilized on Miniature Hydrophobic Columns | p. 399 |
| Enzymatic Digestion of Membrane-Bound Proteins for Peptide Mapping and Internal Sequence Analysis | p. 405 |
| Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels | p. 415 |
| Reverse-Phase HPLC Separation of Enzymatic Digests of Proteins | p. 427 |
| Peptide Mapping by Two-Dimensional Thin-Layer Electrophoresis - Thin-Layer Chromatography | p. 437 |
| Peptide Mapping by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis | p. 447 |
| Peptide Mapping by High-Performance Liquid Chromatography | p. 453 |
| Production of Protein Hydrolysates Using Enzymes | p. 457 |
| Amino Acid Analysis Using Precolumn Derivatization with 6-Aminoquinolyl-n-Hydroxysuccinimidyl Carbamate | p. 461 |
| Amino Acid Analysis Using Precolumn Derivatization with Phenylisothiocyanate | p. 467 |
| Molecular-Weight Estimation for Native Proteins Using Size-Exclusion High-Performance Liquid Chromatography | p. 473 |
| Detection of Disulfide-Linked Peptides by HPLC | p. 479 |
| Diagonal Electrophoresis for Detecting Disulfide Bridges | p. 481 |
| Estimation of Disulfide Bonds Using Ellman's Reagent | p. 487 |
| Quantitation of Cysteine Residues and Disulfide Bonds by Electrophoresis | p. 489 |
| Detection of Disulfide-Linked Peptides by Mass Spectrometry | p. 495 |
| Analyzing Protein Phosphorylation | p. 501 |
| Identification of Proteins Modified by Protein (D-Aspartyl/L-Isoaspartyl) Carboxyl Methyltransferase | p. 507 |
| Analysis of Protein Palmitoylation | p. 517 |
| Removal of Pyroglutamic Acid Residues from the N-Terminus of Peptides and Proteins | p. 525 |
| The Dansyl Method for Identifying N-Terminal Amino Acids | p. 529 |
| The Dansyl-Edman Method for Peptide Sequencing | p. 535 |
| Matrix-Assisted Laser Desorption Ionization Mass Spectrometry as a Complement to Internal Protein Sequencing | p. 541 |
| A Manual C-Terminal Sequencing Procedure for Peptides: The Thiocyanate Degradation Method | p. 557 |
| C-Terminal Sequence Analysis with Carboxypeptidase Y | p. 569 |
| Rapid Epitope Mapping by Carboxypeptidase Digestion and Immunoblotting | p. 573 |
| Epitope Mapping of a Protein Using the Geysen (PEPSCAN) Procedure | p. 581 |
| Epitope Mapping of Protein Antigens by Competition ELISA | p. 595 |
| Identification of Glycoproteins on Nitrocellulose Membranes Using Lectin Blotting | p. 603 |
| A Lectin-Binding Assay for the Rapid Characterization of the Glycosylation of Purified Glycoproteins | p. 619 |
| Staining of Glycoproteins/Proteoglycans on SDS-Gels | p. 627 |
| Chemical Methods of Analysis of Glycoproteins | p. 633 |
| Monosaccharide Analysis by HPAEC | p. 635 |
| Monosaccharide Analysis by GC | p. 639 |
| Determination of Monosaccharide Linkage and Substitution Patterns by GC-MS Methylation Analysis | p. 641 |
| Sialic Acid Analysis by HPAEC-PAD | p. 645 |
| Chemical Release of O-Linked Oligosaccharide Chains | p. 647 |
| O-Linked Oligosaccharide Profiling by HPLC | p. 649 |
| O-Linked Oligosaccharide Profiling by HPAEC-PAD | p. 651 |
| Released of N-Linked Oligosaccharide Chains by Hydrazinolysis | p. 653 |
| Enzymatic Release of O- and N-Linked Oligosaccharide Chains | p. 657 |
| N-Linked Oligosaccharide Profiling by HPLC on Porous Graphitized Carbon (PGC) | p. 659 |
| N-Linked Oligosaccharide Profiling by HPAEC-PAD | p. 661 |
| The Chloramine T Method for Radiolabeling Protein | p. 665 |
| The Lactoperoxidase Method for Radiolabeling Protein | p. 669 |
| The Bolton and Hunter Method for Radiolabeling Protein | p. 671 |
| The Iodogen Method for Radiolabeling Protein | p. 673 |
| Purification and Assessment of Quality of Radioiodinated Protein | p. 675 |
| Conjugation of Peptides to Carrier Proteins via Glutaraldehyde | p. 679 |
| Conjugation of Peptide to Carrier Proteins via m-Maleimidobenzoyl-N-Hydroxysuccinimide Ester (MBS) | p. 689 |
| Conjugation of Peptides to Carrier Protein via Carbodiimide | p. 693 |
| Raising of Polyclonal Antisera | p. 695 |
| Elution of SDS-PAGE Separated Proteins from Immobilon Membranes for Use as Antigens | p. 699 |
| Production of Highly Specific Polyclonal Antibodies Using a Combination of 2D Electrophoresis and Nitrocellulose-Bound Antigen | p. 703 |
| Production and Characterization of Antibodies Against Peptides | p. 711 |
| Production of Antibodies Using Proteins in Gel Bands | p. 717 |
| Purification of IgG by Precipitation with Sodium Sulfate or Ammonium Sulfate | p. 721 |
| Purification of IgG Using Caprylic Acid | p. 723 |
| Purification of IgG Using DEAE-Sepharose Chromatography | p. 725 |
| Purification of IgG Using Ion-Exchange HPLC/FPLC | p. 727 |
| Purification of IgG by Precipitation with Polyethylene Glycol (PEG) | p. 731 |
| Purification of IgG Using Protein A or Protein G | p. 733 |
| Purification of IgG Using Gel-Filtration Chromatography | p. 735 |
| Purification of IgG Using Affinity Chromatography on Antigen-Ligand Columns | p. 739 |
| Purification of IgG Using Thiophilic Chromatography | p. 743 |
| Analysis of IgG Fractions by Electrophoresis | p. 745 |
| Ouchterlony Double Immunodiffusion | p. 749 |
| Single Radial Immunodiffusion | p. 753 |
| Rocket Immunoelectrophoresis | p. 757 |
| Two-Dimensional Immunoelectrophoresis | p. 763 |
| Immunogen Preparation and Immunization Procedures for Rats and Mice | p. 771 |
| Hybridoma Production | p. 773 |
| Screening Hybridoma Culture Supernatants Using Solid-Phase Radiobinding Assay | p. 777 |
| Screening Hybridoma Culture Supernatants Using ELISA | p. 779 |
| Growth and Purification of Murine Monoclonal Antibodies | p. 781 |
| A Rapid Method for Generating Large Numbers of High-Affinity Monoclonal Antibodies from a Single Mouse | p. 783 |
| Index | p. 793 |
| Table of Contents provided by Blackwell. All Rights Reserved. |
책소개
A remarkable treasury of 144 state-of-the-art and highly successful analytical techniques for studying proteins and peptides. John Walker has included many updated and improved methods from his best-selling Basic Protein and Peptide Protocols as well as adding nearly 100 new ones. Each tried-and-tested protocol contains detailed step-by-step instructions time-saving troubleshooting tips alternative procedures informative explanations and comprehensive lists of reagents and suppliers-aids not usually found in standard journal recipes. The techniques do not require sophisticated and expensive apparatus.
저자소개
| 생년월일 | - |
|---|
해당작가에 대한 소개가 없습니다.
주간랭킹
더보기상품정보제공고시
이벤트 기획전
대학교재/전문서적 분야에서 많은 회원이 구매한 책
판매자정보
상호 |
(주)교보문고 |
|---|---|
대표자명 |
안병현 |
사업자등록번호 |
102-81-11670 |
연락처 |
1544-1900 |
전자우편주소 |
callcenter@kyobobook.co.kr |
통신판매업신고번호 |
01-0653 |
영업소재지 |
서울특별시 종로구 종로 1(종로1가,교보빌딩) |
교환/환불
반품/교환 방법 |
‘마이페이지 > 취소/반품/교환/환불’ 에서 신청 또는 1:1 문의 게시판 및 고객센터(1577-2555)에서 신청 가능 |
|---|---|
반품/교환가능 기간 |
변심 반품의 경우 출고완료 후 6일(영업일 기준) 이내까지만 가능 |
반품/교환 비용 |
변심 혹은 구매착오로 인한 반품/교환은 반송료 고객 부담 |
반품/교환 불가 사유 |
·소비자의 책임 있는 사유로 상품 등이 손실 또는 훼손된 경우 ·소비자의 사용, 포장 개봉에 의해 상품 등의 가치가 현저히 감소한 경우 ·복제가 가능한 상품 등의 포장을 훼손한 경우 ·시간의 경과에 의해 재판매가 곤란한 정도로 가치가 현저히 감소한 경우 ·전자상거래 등에서의 소비자보호에 관한 법률이 정하는 소비자 청약철회 제한 내용에 해당되는 경우 |
상품 품절 |
공급사(출판사) 재고 사정에 의해 품절/지연될 수 있음 |
소비자 피해보상 |
·상품의 불량에 의한 교환, A/S, 환불, 품질보증 및 피해보상 등에 관한 사항은 소비자분쟁해결 기준 (공정거래위원회 고시)에 준하여 처리됨 ·대금 환불 및 환불지연에 따른 배상금 지급 조건, 절차 등은 전자상거래 등에서의 소비자 보호에 관한 법률에 따라 처리함 |
(주)KG이니시스 구매안전서비스
(주)인터파크커머스는 회원님들의 안전거래를 위해 구매금액, 결제수단에 상관없이 (주)인터파크커머스를 통한 모든 거래에 대하여
(주)KG이니시스가 제공하는 구매안전서비스를 적용하고 있습니다.
배송안내
교보문고 상품은 택배로 배송되며, 출고완료 1~2일내 상품을 받아 보실 수 있습니다.
출고가능 시간이 서로 다른 상품을 함께 주문할 경우 출고가능 시간이 가장 긴 상품을 기준으로 배송됩니다.
군부대, 교도소 등 특정기관은 우체국 택배만 배송가능합니다.
배송비는 업체 배송비 정책에 따릅니다.
- 도서 구매 시 15,000원 이상 무료배송, 15,000원 미만 2,500원
- 상품별 배송비가 있는 경우, 상품별 배송비 정책 적용